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licor intercept blocking buffer  (LI-COR)


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    Structured Review

    LI-COR licor intercept blocking buffer
    Licor Intercept Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/licor+intercept+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/pmc13110130-283-50-54
    Average 98 stars, based on 2114 article reviews
    licor intercept blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Atypical antipsychotics alter microglial functions via astrocyte-derived extracellular vesicles
    Article Snippet: .. Membranes were blocked with LICOR Intercept Blocking Buffer (LI-COR, #927-70001) for 1 hour at room temperature. .. Primary antibodies (Rabbit anti-CD9, Abcam, #ab263019; Rabbit anti-CD81, Abcam, #ab109201; Rabbit anti-TSG101, Abcam, #ab125011; Rabbit anti-Calnexin, Abcam, #ab133615) were diluted 1:1000 in Blocking Buffer and 0.2% Tween-20 (Sigma, #P2287) and incubated overnight at 4°C.

    Article Title: Multi-omic phenotyping of MAPT V337M neurons reveals early changes in axonogenesis and tau phosphorylation
    Article Snippet: The supernatants were collected, and the concentrations were measured with the BCA assay (Thermo Fisher Scientific; Cat No. 23225). .. 10–20 μg protein were loaded onto 4–12% Bis-Tris polyacrylamide gel (Thermo Fisher Scientific; Cat No. NP0336BOX) Nitrocellulose (BioRad, Cat. No. 1620146) or PVDF membranes were used to transfer the protein in a BioRad Transblot for 11 minutes at 25 V, 25 A. Membranes were then blocked for 1 hour with Licor Intercept blocking buffer (Licor, Cat. No. 927-60001) at room temperature. .. Primary antibody was added in Licor Intercept block overnight at 4 °C.

    Article Title: Circulating Tumor Cells Shed Shearosome Extracellular Vesicles in Capillary Bifurcations That Activate Endothelial and Immune Cells
    Article Snippet: .. The membrane was briefly rinsed in tris‐buffered saline (TBS) and blocked in a solution consisting of 5 mL Licor Intercept Blocking Buffer (Licor, 927–60001) and 5 mL ultrapure water for 1 h at room temperature on a rocker. .. Primary antibody incubation was performed overnight at 4 °C to detect HSP90 and CD81 as previously, using a solution containing 5 mL Licor Intercept Blocking Buffer, 5 mL ultrapure water, and 10 μL Tween‐20 (Sigma‐Aldrich, P7949).

    Article Title: GLP-1R/GCGR dual agonism dissipates hepatic steatosis to restore insulin sensitivity and rescue pancreatic β-cell function in obese male mice
    Article Snippet: Samples were dry transferred to nitrocellulose membrane using the IBlot 2 system and reagents (Thermo, Ref #IB21001, #IB23002). .. Membranes were blocked for 1 h in Licor Intercept Blocking Buffer (Ref #927-60001) and then incubated in primary antibodies for total Akt (Cell Signaling, Ref #9272S, 1:1000), phospho-Akt Serine 473 (Cell Signaling, Ref # 4060S, 1:1000), or phospho-Akt Threonine 308 (Cell Signaling, Ref #13038, 1:1000) diluted in Licor Intercept Protein-Free Antibody Diluent (Ref #927-85001) at 4 °C overnight. .. Membranes were washed in TBST 10 min, 3X, and then incubated in Licor Goat Anti-rabbit secondary antibodies diluted 1:20,000 in Licor Intercept Protein-Free Antibody Diluent for 1 h. Membranes were washed in TBS 10 min, 3X and then imaged on a Licor Odyssey Imager.

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans.
    Article Snippet: .. Primary antibodies were incubated in a 3:1 solution of LiCor-Intercept blocking buffer (TBS version) in TBST (Tris-buffered saline + 0.1 % Tween 20). ..

    Article Title: GLP-1R/GCGR dual agonism dissipates hepatic steatosis to restore insulin sensitivity and rescue pancreatic β-cell function in obese male mice.
    Article Snippet: Samples were dry transferred to nitrocellulose membrane using the IBlot 2 system and reagents (Thermo, Ref #IB21001, #IB23002). .. Membranes were blocked for 1 h in Licor Intercept Blocking Buffer (Ref #927-60001) and then incubated in primary antibodies for total Akt (Cell Signaling, Ref #9272S, 1:1000), phospho-Akt Serine 473 (Cell Signaling, Ref # 4060S, 1:1000), or phospho-Akt Threonine 308 (Cell Signaling, Ref #13038, 1:1000) diluted in Licor Intercept Protein-Free Antibody Diluent (Ref #927-85001) at 4 °C overnight. .. Membranes were washed in TBST 10min, 3X, and then incubated in Licor Goat Anti-rabbit secondary antibodies diluted 1:20,000 in Licor Intercept Protein-Free Antibody Diluent for 1 h. Membranes were washed in TBS 10min, 3X and then imaged on a Licor Odyssey Imager.

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans
    Article Snippet: .. Primary antibodies were incubated in a 3:1 solution of LiCor-Intercept blocking buffer (TBS version) in TBST (Tris-buffered saline + 0.1% Tween 20). ..

    Article Title: Vasomotor responses are similar between outbred UM-HET3 and inbred C57BL/6J male and female mouse mesenteric resistance arteries
    Article Snippet: The amount of protein loaded on the SDS-PAGE gels was ∼40 μg/sample, which was estimated based on measuring protein concentration using the Lowry method (DC Protein Assay, Bio-Rad, Hercules, CA) in only a subset of samples to preserve limited protein. .. Membranes were then blocked for 1 h at room temperature using a LiCor Intercept Blocking Buffer (#927-70001, LiCor, Lincoln, NE) and incubated overnight with an eNOS antibody (BD Transduction Laboratories, Franklin Lakes, NJ, #610297, mouse monoclonal, 1:1,000) diluted in LiCor Intercept Antibody Diluent (#927-75001), followed by four washes of 5 min each with PBS +0.1% Tween-20. .. Subsequently, the membranes were incubated for 45 min with a near-infrared secondary antibody [goat anti-mouse IRDye 800 CW (#926-32210), 1:20,000, LiCor] diluted in LiCor Intercept Antibody Diluent, then washed again four times for 5 min each with PBS +0.1% Tween-20, and finally rinsed and stored in PBS.

    Membrane:

    Article Title: Circulating Tumor Cells Shed Shearosome Extracellular Vesicles in Capillary Bifurcations That Activate Endothelial and Immune Cells
    Article Snippet: .. The membrane was briefly rinsed in tris‐buffered saline (TBS) and blocked in a solution consisting of 5 mL Licor Intercept Blocking Buffer (Licor, 927–60001) and 5 mL ultrapure water for 1 h at room temperature on a rocker. .. Primary antibody incubation was performed overnight at 4 °C to detect HSP90 and CD81 as previously, using a solution containing 5 mL Licor Intercept Blocking Buffer, 5 mL ultrapure water, and 10 μL Tween‐20 (Sigma‐Aldrich, P7949).

    Saline:

    Article Title: Circulating Tumor Cells Shed Shearosome Extracellular Vesicles in Capillary Bifurcations That Activate Endothelial and Immune Cells
    Article Snippet: .. The membrane was briefly rinsed in tris‐buffered saline (TBS) and blocked in a solution consisting of 5 mL Licor Intercept Blocking Buffer (Licor, 927–60001) and 5 mL ultrapure water for 1 h at room temperature on a rocker. .. Primary antibody incubation was performed overnight at 4 °C to detect HSP90 and CD81 as previously, using a solution containing 5 mL Licor Intercept Blocking Buffer, 5 mL ultrapure water, and 10 μL Tween‐20 (Sigma‐Aldrich, P7949).

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans.
    Article Snippet: .. Primary antibodies were incubated in a 3:1 solution of LiCor-Intercept blocking buffer (TBS version) in TBST (Tris-buffered saline + 0.1 % Tween 20). ..

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans
    Article Snippet: .. Primary antibodies were incubated in a 3:1 solution of LiCor-Intercept blocking buffer (TBS version) in TBST (Tris-buffered saline + 0.1% Tween 20). ..

    Incubation:

    Article Title: GLP-1R/GCGR dual agonism dissipates hepatic steatosis to restore insulin sensitivity and rescue pancreatic β-cell function in obese male mice
    Article Snippet: Samples were dry transferred to nitrocellulose membrane using the IBlot 2 system and reagents (Thermo, Ref #IB21001, #IB23002). .. Membranes were blocked for 1 h in Licor Intercept Blocking Buffer (Ref #927-60001) and then incubated in primary antibodies for total Akt (Cell Signaling, Ref #9272S, 1:1000), phospho-Akt Serine 473 (Cell Signaling, Ref # 4060S, 1:1000), or phospho-Akt Threonine 308 (Cell Signaling, Ref #13038, 1:1000) diluted in Licor Intercept Protein-Free Antibody Diluent (Ref #927-85001) at 4 °C overnight. .. Membranes were washed in TBST 10 min, 3X, and then incubated in Licor Goat Anti-rabbit secondary antibodies diluted 1:20,000 in Licor Intercept Protein-Free Antibody Diluent for 1 h. Membranes were washed in TBS 10 min, 3X and then imaged on a Licor Odyssey Imager.

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans.
    Article Snippet: .. Primary antibodies were incubated in a 3:1 solution of LiCor-Intercept blocking buffer (TBS version) in TBST (Tris-buffered saline + 0.1 % Tween 20). ..

    Article Title: GLP-1R/GCGR dual agonism dissipates hepatic steatosis to restore insulin sensitivity and rescue pancreatic β-cell function in obese male mice.
    Article Snippet: Samples were dry transferred to nitrocellulose membrane using the IBlot 2 system and reagents (Thermo, Ref #IB21001, #IB23002). .. Membranes were blocked for 1 h in Licor Intercept Blocking Buffer (Ref #927-60001) and then incubated in primary antibodies for total Akt (Cell Signaling, Ref #9272S, 1:1000), phospho-Akt Serine 473 (Cell Signaling, Ref # 4060S, 1:1000), or phospho-Akt Threonine 308 (Cell Signaling, Ref #13038, 1:1000) diluted in Licor Intercept Protein-Free Antibody Diluent (Ref #927-85001) at 4 °C overnight. .. Membranes were washed in TBST 10min, 3X, and then incubated in Licor Goat Anti-rabbit secondary antibodies diluted 1:20,000 in Licor Intercept Protein-Free Antibody Diluent for 1 h. Membranes were washed in TBS 10min, 3X and then imaged on a Licor Odyssey Imager.

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans
    Article Snippet: .. Primary antibodies were incubated in a 3:1 solution of LiCor-Intercept blocking buffer (TBS version) in TBST (Tris-buffered saline + 0.1% Tween 20). ..

    Article Title: Vasomotor responses are similar between outbred UM-HET3 and inbred C57BL/6J male and female mouse mesenteric resistance arteries
    Article Snippet: The amount of protein loaded on the SDS-PAGE gels was ∼40 μg/sample, which was estimated based on measuring protein concentration using the Lowry method (DC Protein Assay, Bio-Rad, Hercules, CA) in only a subset of samples to preserve limited protein. .. Membranes were then blocked for 1 h at room temperature using a LiCor Intercept Blocking Buffer (#927-70001, LiCor, Lincoln, NE) and incubated overnight with an eNOS antibody (BD Transduction Laboratories, Franklin Lakes, NJ, #610297, mouse monoclonal, 1:1,000) diluted in LiCor Intercept Antibody Diluent (#927-75001), followed by four washes of 5 min each with PBS +0.1% Tween-20. .. Subsequently, the membranes were incubated for 45 min with a near-infrared secondary antibody [goat anti-mouse IRDye 800 CW (#926-32210), 1:20,000, LiCor] diluted in LiCor Intercept Antibody Diluent, then washed again four times for 5 min each with PBS +0.1% Tween-20, and finally rinsed and stored in PBS.



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